Vesicle fusion to planar membranes is enhanced by cholesterol and low temperature

DE Lee, MG Lew, DJ Woodbury - Chemistry and physics of lipids, 2013 - Elsevier
… composed of artificial vesicles (liposomes) and artificial membranes (planar lipid bilayers),
we observed that fusion is significantly affected by the lipid phase of the planar membrane. To …

Lipid vesicles and membrane fusion

G Cevc, H Richardsen - Advanced Drug Delivery Reviews, 1999 - Elsevier
temperature of DOPE. This effect is eliminated by the addition of membrane-stiffening cholesterol,
which … Moreover, osmotic swelling of the vesicles contacting the planar membrane puts …

[HTML][HTML] Modulation of lipid vesiclemembrane interactions by cholesterol

CP Chng, KJ Hsia, C Huang - Soft Matter, 2022 - pubs.rsc.org
… of cholesterol in either the vesicle or planar membrane accelerates membrane fusion and
… is the mean square fluctuation, k B is the Boltzmann constant and T is the temperature. The …

[PDF][PDF] Vesicle-Membrane Fusion is Enhanced by Cholesterol and Low Temperature

DJ Woodbury - Biophysical Journal, 2012 - cell.com
… important role in fusion of vesicles to cell membranes, an essential … vesicles and planar
lipid membranes, we observed that fusion is significantly affected by the lipid phase of the planar

Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. II. Incorporation of a vesicular membrane marker into the planar membrane.

FS Cohen, J Zimmerberg, A Finkelstein - The Journal of general …, 1980 - rupress.org
… in the cis compartment are enhanced orders of magnitude by … Planar membranes were
formed at room temperature (22-24… paper differs from biological vesicle-membrane fusion, as it …

Membrane fusion and molecular segregation in phospholipid vesicles

D Papahadjopoulos, G Poste, BE Schaeffer… - Biochimica et biophysica …, 1974 - Elsevier
… the presence of cholesterol on the fusion reaction and the … the endothermic peak toward
lower temperatures. In this case… to lateral diffusion on the plane of the membranes would be very …

Membrane fusion correlates with surface charge in exocytic vesicles

JG Duman, E Lee, GY Lee, G Singh, JG Forte - Biochemistry, 2004 - ACS Publications
… was able to enhance tubulovesicle−tubulovesicle fusion in vitro; thus… at very low temperatures
where the very low rates created … to an electric field is greatly stabilized by their cholesterol

[HTML][HTML] Vesicle cholesterol controls exocytotic fusion pore

B Rituper, A Guček, M Lisjak, U Gorska, A Šakanović… - Cell Calcium, 2022 - Elsevier
… Whether increased vesicle cholesterol affects vesicle fusion … min at room temperature (RT).
To immunolabel PRL secretory … positive vesicles per cell plane (C) and the cumulative vesicle

Membrane and vesicle fusion

JH Prestegard, MP O'Brien - Annual Review of Physical …, 1987 - annualreviews.org
… Also used are planar bilayers, which are simply a flat sheet of … isotropic phases were noted
in a temperature-dependent study with vesicles containing PE, PC, and cholesterol in a 3 : I : …

Quantitative description of the vesicle fusion mechanism on solid surfaces and the role of cholesterol

S Abraham, T Heckenthaler… - The Journal of …, 2018 - ACS Publications
… nm pore size membrane ∼10 times (at a temperature of ∼55 … vesicle fusion at 45 C, R̂
LB increased from 1.2 to 22 kΩ cm 2 for lipids with no cholesterol and with 50 mol % cholesterol, …